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GPLS5000 is a skin-soothing cosmetic composition containing the mixed extracts
of gynostemma pentaphyllum, saponaria officinalis and Lonicera japonica as active components.
GPLS5000S under Patent No. 10-2364260 is a skin-soothing cosmetic composition containing the mixed extracts of gynostemma pentaphyllum, saponaria officinalis and Lonicera japonica as active components.
(Gynostemma pentaphyllum, Lonicera japonica Flower, Saponaria officinalis Leaf)
As the combination of extracts of gynostemma pentaphyllum, saponaria officinalis, and Lonicera japonica whose efficacy has been proved through a variety of papers and research results, it is the patented ingredient of aazzits that enhances the saponin component of ginseng, which has been used medicinally for its anti-oxidation and anti-cancer effects. Please find our aazzits Calming Ampoule
Efficacy test and results of GPLS5000
[Registered Patent Publication]
The stratum corneum of the skin is the first barrier between the skin and the external environment, and is an important factor in determining the condition of the skin.
The stratum corneum of healthy skin contains 15-20% of water, and when the water content falls below 10%, it becomes dry and loses luster and elasticity, resulting in increased wrinkles. When defects occur due to extrinsic factors such as penetration of irritants or endogenous factors such as aging, hormones, and stress, resistance decreases, resulting in deterioration of the epidermal double lipid film and increased sensitivity to irritants, resulting in sensitive and dry skin.
In general, skin is constantly stimulated by external influences such as cosmetics, stress, and air pollution for a long period of time, resulting in increased sensitivity and becoming sensitive skin that causes dermatitis. The main symptoms of sensitive skin include redness, skin dryness, dermatitis, and acne. Among them, redness is a phenomenon in which the skin becomes red and hot, and the skin is reddened and congested due to the expansion of blood vessels. It is the most common skin reaction caused by irritation.
In addition, as the skin barrier function is weakened by stimulation, irritation-inducing substances penetrate into the stratum corneum and cause irritation, resulting in skin inflammation. Here is skin barrier recovery ampoule from Dr.A global
Under this background, the study has been conducted to develop cosmetics with a skin soothing effect, and a mixed extract prepared by mixing gynostemma pentaphyllum, saponaria officinalis, and Lonicera japonica at a specific ratio, which have been used as conventional ingredients for cosmetics, has excellent effects of improving skin inflammation and skin irritation due to its synergistic effect. aazzitsCalming ampoule' ingredient has been completed by confirming that it exhibits not only the relief and soothing effects but also excellent skin moisturizing and skin barrier strengthening effects with GPLS5000.
1. Result of confirming the effect of GPLS5000 protecting skin cells
The combination of the extracts of gynostemma pentaphyllum, saponaria officinalis and Lonicera japonica (Fifth example embodiment) was confirmed to have the excellent cell protecting effects from UV stimulation compared to the comparative examples of extracts of gynostemma pentaphyllum, saponaria officinalis and Lonicera japonica, and comparative examples with different contents.
Test example1. Result of confirming the effect of GPLS5000 protecting skin cells
| Division | Treatment concentration | Cell viability(%) |
|---|---|---|
| Example1 | 0.1 | 66.2 |
| Example2 | 0.1 | 65.6 |
| Example3 | 0.1 | 69.3 |
| Example4 | 0.1 | 72.8 |
| Example5 | 0.1 | 76.1 |
| Example6 | 0.1 | 71.2 |
| Comparative example1 | 0.1 | 58.7 |
was confirmed to have the excellent cell protecting effects from UV stimulation compared to the comparative examples of extracts of gynostemma pentaphyllum, saponaria officinalis and Lonicera japonica, and comparative examples with different contents.
2. The effect of reducing the expression of inflammatory factors by stimulation
The combination of the extracts of gynostemma pentaphyllum, saponaria officinalis and Lonicera japonica (Fifth example embodiment)Compared to Comparative Examples of extracts of a single natural substance and Comparative Examples with different contents, the expression of inflammatory factors induced by stimulation was further reduced, and excellent effects were exhibited.
Test example2. Reduced expression of COX-2 and PGE-2 by stimulation
| Division | COX-2 expression reduction rate | PEG2 expression reduction rate | ||||
|---|---|---|---|---|---|---|
| UV-B | LPS | SLS | UV-B | LPS | SLS | |
| Example2 | 16.0 | 11.0 | 22.5 | 17.4 | 10.2 | 9.8 |
| Example3 | 18.8 | 15.8 | 19.0 | 15.5 | 9.9 | 10.3 |
| Example4 | 21.4 | 13.4 | 25.4 | 18.6 | 13.4 | 11.1 |
| Example5 | 28.1 | 21.2 | 28.2 | 22.7 | 18.9 | 17.0 |
| Example6 | 22.3 | 16.8 | 24.8 | 20.3 | 15.0 | 12.9 |
| Comparative example1 | 14.4 | 10.7 | 14.3 | 14.1 | 9.4 | 8.2 |
The combination of the extracts of gynostemma pentaphyllum, saponaria officinalis and Lonicera japonica Compared to Comparative Example, which is an extract of a single natural product, the expression of COX-2 and PGE-2, which are inflammatory factors induced by stimulation, was further reduced, and Example 5 showed the best effect.
Test example3. Reduced expression of NF-kB and TNF-a by stimulation
| Division | NF-kb expression reduction rate | TNF-a expression reduction rate | ||||
|---|---|---|---|---|---|---|
| UV-B | LPS | SLS | UV-B | LPS | SLS | |
| Example2 | 18.6 | 10.1 | 17.3 | 15.1 | 13.2 | 12 |
| Example3 | 20.8 | 13.7 | 14.4 | 17.2 | 19.8 | 14.5 |
| Example4 | 22 | 19.4 | 18.9 | 19.9 | 22.6 | 17.3 |
| Example5 | 28.4 | 21.8 | 22.3 | 25.8 | 29.3 | 21.4 |
| Example6 | 24.3 | 18.7 | 21.2 | 23 | 25.4 | 19.9 |
| Comparative example1 | 16.7 | 9.4 | 11.8 | 13 | 9.3 | 8.8 |
The combination of the extracts of gynostemma pentaphyllum, saponaria officinalis and Lonicera japonica mixed extract further reduced the expression of irritation-induced inflammatory factors NF-kb and TNF-a compared to Comparative Example, which is an extract of a single natural product.
3. Measurement of hyaluronic acid production by GPLS5000
The combination of the extracts of gynostemma pentaphyllum, saponaria officinalis and Lonicera japonica (Fifth example embodiment) Compared to the comparative example of the extract of a single natural substance and that with different contents, it showed the excellent hyaluronic acid production, and the excellent effects.*GPLS5000 Calming ampoule
| Division | HA production amount(ug/ml) |
|---|---|
| Example1 | 30.4 |
| Example2 | 33.7 |
| Example3 | 36.2 |
| Example4 | 40.6 |
| Example5 | 43 |
| Example6 | 38.6 |
| Comparative example1 | 28.3 |
The combination of the extracts of gynostemma pentaphyllum, saponaria officinalis and Lonicera japonica (Fifth example embodiment)Compared to the comparative example of the extract of a single natural substance and that with different contents, it showed the excellent hyaluronic acid production, and the excellent effects.
4. Confirming the effect of GPLS5000 on reducing skin redness
The combination of the extracts of gynostemma pentaphyllum, saponaria officinalis and Lonicera japonica (Seventh example embodiment) showed the more excellent effect of alleviating skin redness than the composition of the first control example.*aazzits : Calming ampoule and skin barrier recovery ampoule
| Division | Initial average | 2-week average | Improvement rate(%) |
|---|---|---|---|
| Control example1 | 10.92 | 10.47 | 4.12 |
| Example7 | 11.28 | 10.22 | 9.40 |
The combination of the extracts of gynostemma pentaphyllum, saponaria officinalis and Lonicera japonica (Seventh example embodiment)showed the more excellent effect of alleviating skin redness than the composition of the first control example.
5. Confirming the effect of strengthening the skin barrier function of GPLS5000
The cream containing the mixed extracts of gynostemma pentaphyllum, saponaria officinalis and Lonicera japonica (7th example embodiment) showed the excellent effect of strengthening the skin barrier function compared to the control example 1 that does not contain them.
* Comparison of changes in water loss over time based on epidermal water loss (100%) (the lower the number, the higher the effect)
| Division | 0 hour | 2 hour | 4 hour | 6 hour | 8 hour |
|---|---|---|---|---|---|
| Control example1 | 100 | 45 | 61 | 75 | 85 |
| Example7 | 100 | 27 | 36 | 48 | 57 |
The cream containing the mixed extracts of gynostemma pentaphyllum, saponaria officinalis and Lonicera japonica (7th example embodiment)showed the excellent effect of strengthening the skin barrier function compared to the control example 1 that does not contain them.
Method of extracting the ingredients of GPLS5000
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Collect the carefully selected ingredients, and dry the collected ingredients.
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Ground the dried ingredients and then extract them by using the low-temperature extraction method.
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After concentrating the extracted ingredients, freeze-dry and produce the powder of gynostemma pentaphyllum at 99% high concentration and high purity.
Maintaining the effective components by making it as high-quality, highly concentrated powder, it delivers the reliable effect by raising the content.
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Gynostemma pentaphyllum, key ingredient of GPLS5000
Gynostemma pentaphyllum, which is called the herb of immortality, is a native plant of Ulleungdo Island and has already proven its effectiveness and efficacy through many papers. According to experiments and studies, it contains more than 40 times the same saponin as 4 types of ginseng, including 100 types of saponins composed of ingredients similar to ginseng, so it is excellent in improving immunity, protecting skin, and soothing skin, helping in relieving chronic itching and restoring skin barrier function. In addition, by discovering the effect of enhancing collagen synthesis through an experiment with the extract of gynostemma pentaphyllum, it was proved that it is effective in reducing wrinkles through the improvement of the aging of inner skin that proceeds first.
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Lonicera japonica, key components of GPLS5000
Lonicera japonica which is contained aazzits calming ampouleis also used for inflammatory diseases and is already widely used as a medicine in oriental medicine, such as anti-inflammatory, anti-viral, antioxidant, and immunity enhancing effects. In addition, through research to show efficacy in the human body, it has been proven to be effective in brightening by helping to inhibit tyrosinase, a melanin-producing enzyme, and proved to be excellent in antioxidant effects due to its high tocopherol and flavonoid content.
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Saponaria officinalis, key components of GPLS5000
Saponaria officinalis is a natural surfactant containing a high content of saponin in the calming ampoule and is known as a treatment applied to problematic skin such as eczema, ringworm, and acne, and has antifungal and anti-inflammatory effects on the skin.
